Light microscopes had several drawbacks, which led to the invention of the electron microscope. Photon diffraction was the basis of light microscopes.
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The concept of the electron microscope was born when it was discovered that it is also possible to achieve diffraction using electrons.
The wavelength of electrons is significantly less than that of photons because electrons have a significantly higher mass than protons.
As a result, electrons diffract less than photons. An electron microscope uses this principle so that it can produce sharper images that can be magnified much more than a light microscope.
There are two types of electron microscopes most commonly used: scanning electron microscopes (SEM) and transmission electron microscopes (TEM).
Scanning Electron Microscope vs Transmission Electron Microscope
The main difference between Scanning Electron Microscope and Transmission Electron Microscope is that scanning electron microscopes produce surface images by reflecting electrons from the specimen’s surface, while transmission electron microscopes produce an internal image of the specimen by emitting electrons that cross through it.
An SEM ejects a fine and focused beam of electrons towards a sample. These electrons are captured after reflecting from the specimen’s surface.
A magnified image is created when electrons interact with the specimen’s surface. A magnified version of the specimen’s surface can be produced up to 2,000,000 times.
A TEM is an electron microscope that emits a broad beam of electrons. Electrons are captured after passing through this beam, which penetrates the entire specimen.
Images of the specimen are thus obtained showing its detailed internal structure. This image can be magnified up to 50,000,000 times.
Comparison Table Between Scanning Electron Microscope and Transmission Electron Microscope
|Parameters of Comparison||Scanning Electron Microscope||Transmission Electron Microscope|
|Definition||Electrons ejected by SEMs are reflected from the surface itself, that is, they do not penetrate the specimen.||Electrons ejected by TEMs pass through the entire specimen, that is, they do penetrate it.|
|Image Produced||SEMs produce topographical or surface images of specimens.||TEMs provide detailed internal images of the specimen.|
|Electrons ejected||Fine and focused electron beams are emitted by SEMs.||Electrons are emitted from TEMs in a broad beam.|
|Resolution||The images produced have a low resolution.||The images produced have a high resolution.|
|Image dimensions||Only 2D images can be produced by SEMs.||3D images can be produced by TEMs.|
|Speed||SEMs are faster at processing images.||TEMs process images at a slower rate.|
|Magnification||SEMs have a magnification power of up to 2,000,000.||The magnification power of TEMs can reach 50,000,000 times.|
|Sample preparation||For magnification, SEMs do not require much sample preparation.||It is necessary to prepare the sample for magnification in TEMs.|
|Cost||The cost of operating SEMs is low.||TEMs have high operating costs.|
|Skills required||SEMs are easy to use and don’t require specialized skills to run.||TEMs are complicated devices that require some level of training to operate.|
What is a Scanning Electron Microscope?
A scanning electron microscope (SEM) employs electrons to illuminate a specimen and produce an enlarged image. It uses an electron gun to eject electrons from the specimen.
An electron beam is emitted from the gun. SEM emits electrons in a fine and focused beam.
As compared to a light microscope, which can also magnify substantially, SEM can create a higher resolution image.
The electrons ejected by SEMs are reflected from the surface itself, so they do not penetrate the specimen. Using an SEM, specimens can be imaged topographically or surface-wise.
SEMs emit fine and focused electron beams. The images they produce are of low resolution.
SEMs can produce only two-dimensional images. These devices process images more quickly.
Magnification powers of SEMs can reach up to 2,000,000. SEMs do not require much sample preparation before magnification.
They are relatively inexpensive to operate. Search engine marketing is easy to use and does not require specialized skills to operate.
What is a Transmission Electron Microscope?
Another type of electron microscope is the transmission electron microscope.
Its resolution power is much greater than a photon projector because it creates images by projecting electrons rather than photons.
TEMs emit electron beams in the form of broad beams of electrons. The electrons ejected by TEMs pass through the entire specimen, so they do penetrate it.
These images give a detailed view of the internal structure of the specimen.
There is a high degree of resolution in the images produced. It is possible to produce 3D images with TEMs.
The magnification power of TEMs reaches 50,000,000 times. TEMs process images more slowly.
In TEMs, the sample must be prepared for magnification. The operating costs of TEMs are high.
There is some training required to operate TEMs since they are complicated devices.
Main Differences Between Scanning Electron Microscope and Transmission Electron Microscope
- SEMs emit fine and focused electron beams that are reflected from the surface of the specimen, whereas TEMs emit electrons in a broad beam that passes through the entire specimen, thus penetrating it.
- SEMs produce low-resolution topographic or surface images of specimens, whereas TEMs produce high-resolution, detailed images of the specimen’s interior.
- The SEM processes images faster and has a magnification power of up to 2,000,000, while the TEM processes images at a slower rate and has a magnification power of up to 50,000,000.
- SEMs have a low operating cost, while TEMs have a high operating cost.
- A SEM is easy to use and does not require specific skills to run, while a TEM is a complex device that requires some level of training to operate.
Both the scanning electron microscope and transmission electron microscope are high-resolution microscopes that have a lot of things in common.
They both contain an electron gun that is used to eject electrons towards a sample that is kept in a vacuum.
They each also have lenses and apertures that are used to capture electrons and produce images.
Nevertheless, the difference between both these electron microscopes is vast. SEMs are preferred when the sample is a little large and only the surface details are required.
TEMs are preferred when the samples are very small and a detailed internal is desired.
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